Journal: British Journal of Cancer

Article Title: Treatment with interleukin-2 in malignant pleural mesothelioma: immunological and angiogenetic assessment and prognostic impact

doi: 10.1038/sj.bjc.6605438

Figure Lengend Snippet: Correlations between immunological and angiogenetic parameters in the IL-2-treated patients’ group and in the untreated patients’ group

Article Snippet: For TIL-immunohistochemical staining, sections were incubated with the following antibodies: mouse anti-human Foxp3 monoclonal antibody (clone 236A/E7 diluted 1 : 300; Abcam, Cambridge, UK) , mouse anti-human CD8 monoclonal antibody (clone C8/144B, ready to use for the Ventana automated slide stainer; Ventana) , and mouse anti-human CD4 monoclonal antibody (clone 1F6 diluted 1 : 20; Diagnostic BioSystem, Pleasanton, CA, USA).

Techniques:

Journal: British Journal of Cancer

Article Title: Treatment with interleukin-2 in malignant pleural mesothelioma: immunological and angiogenetic assessment and prognostic impact

doi: 10.1038/sj.bjc.6605438

Figure Lengend Snippet: Association of immunological and angiogenetic variables with clinicopathologic parameters

Article Snippet: For TIL-immunohistochemical staining, sections were incubated with the following antibodies: mouse anti-human Foxp3 monoclonal antibody (clone 236A/E7 diluted 1 : 300; Abcam, Cambridge, UK) , mouse anti-human CD8 monoclonal antibody (clone C8/144B, ready to use for the Ventana automated slide stainer; Ventana) , and mouse anti-human CD4 monoclonal antibody (clone 1F6 diluted 1 : 20; Diagnostic BioSystem, Pleasanton, CA, USA).

Techniques:

Journal: British Journal of Cancer

Article Title: Treatment with interleukin-2 in malignant pleural mesothelioma: immunological and angiogenetic assessment and prognostic impact

doi: 10.1038/sj.bjc.6605438

Figure Lengend Snippet: Univariate analysis of overall survival and time to progression in MPM patients treated with IL-2

Article Snippet: For TIL-immunohistochemical staining, sections were incubated with the following antibodies: mouse anti-human Foxp3 monoclonal antibody (clone 236A/E7 diluted 1 : 300; Abcam, Cambridge, UK) , mouse anti-human CD8 monoclonal antibody (clone C8/144B, ready to use for the Ventana automated slide stainer; Ventana) , and mouse anti-human CD4 monoclonal antibody (clone 1F6 diluted 1 : 20; Diagnostic BioSystem, Pleasanton, CA, USA).

Techniques:

Journal: Scientific Reports

Article Title: Effects of different conditioning regimens on HLA-mismatched microtransplantation and changes in fine immune indices in acute myeloid leukaemia

doi: 10.1038/s41598-024-70332-7

Figure Lengend Snippet: Experimental reagents.

Article Snippet: APC-labelled Mouse Anti-Human CD4 Antibody , BD Bioscience, USA , 662965.

Techniques:

Journal: Journal for Immunotherapy of Cancer

Article Title: CD70 as an actionable immunotherapeutic target in recurrent glioblastoma and its microenvironment

doi: 10.1136/jitc-2021-003289

Figure Lengend Snippet: Modeling of CD70s influence on GBM TIME. (A) CD70 expression kinetics on in-house, activated T cells and (B) levels of CD69 and cMyc displayed by CD70CAR or ConCAR-T cells 9 days post-transduction, evaluated by flow cytometry. (C) CD70-enriched or -silenced Jurkat cells were transduced with either ConCAR or CD70CAR. After 8 days, CD69 and CD70 levels were assessed by flow cytometry. (D) TIME cells extracted from patient tumor samples were analyzed by flow cytometry, evaluating the pattern of expression of CD27 in non-lymphoid (CD45+CD3-) and M1 populations (CD45+CD3-CD68+HLADR+). (E) Average expression of CD27 on CD4/CD8 lymphoid population, and CD70 expression on the lymphoid population (CD3+). CAR, chimeric antigen receptor; GBM, glioblastoma; TIME, tumor immune microenvironment.

Article Snippet: Antibodies used are as follows and were used according to manufacturer’s protocol: PE-Cy7 Mouse Anti-Human CD3 (Cat 563423; BD Pharmingen), PE Mouse Anti-Human CD4 (Cat555347; BD Pharmingen), APC Mouse Anti-Human CD8 (Cat555369; BD Pharmingen), PE-CF594 Mouse Anti-Human CD68 (Cat564944; BD Horizon), APC-H7 Mouse Anti-Human HLA-DR (Cat561358; BD Pharmingen), BV421 Mouse Anti-Human CD27 (Cat562514; BD Horizon).

Techniques: Expressing, Transduction, Flow Cytometry

Journal: Lab animal

Article Title: Generation of the NeoThy mouse model for human immune system studies

doi: 10.1038/s41684-023-01196-z

Figure Lengend Snippet: Flow Cytometry Engraftment Analysis. Gating strategy is shown for determination of common human T cell markers. Viable single human CD45+ cells are subgated into human CD3+ T cell subsets of interest, including CD4+ and CD8+ cells, as well as naïve (CD45RA+CCR7+), effector (CD45RA+CCR7−), central memory (CD45RA−CCR7+), and effector memory (CD45RA−CCR7−) CD8+ T cells.

Article Snippet: Live Dead Fixable Blue (Thermo Fisher Scientific, cat. no. L23105)Flow Cytometry Antibodies (all 5μl per test unless noted) APC Mouse Anti-Human CD34 (BD Pharmingen, cat. no. 561209)PerCP Rat Anti-Mouse CD45 (mLy5) (BD Pharmingen, cat. no. 557235) 1μl/testPerCP Anti-Human CD19 (BD Pharmingen, cat. no. 345778) 5μl/testBV605 Mouse Anti-Human CD3 (BD Pharmingen, cat. no. 564712)APC-Cy7 Mouse Anti-Human CD45 (Biolegend, cat. no. 304014)AmCyan Mouse Anti-Human CD4 (BD Pharmingen, cat. no. 339187)APC Mouse Anti-Human CD8 (BD Pharmingen, cat. no. 566853)FITC Mouse Anti-Human CD45RA (BD Pharmingen, cat. no. 347723)Pacific Blue Mouse Anti-Human CCR7 (Biolegend, cat. no.353209).

Techniques: Flow Cytometry

Journal: The Journal of Experimental Medicine

Article Title: Cell circuits between B cell progenitors and IL-7 + mesenchymal progenitor cells control B cell development

doi: 10.1084/jem.20180778

Figure Lengend Snippet: Relationship between enforced positioning within IL-7 + niches, RAG2 protein stability, and preB cell developmental progression. (A) Overexpression of FAK ( Ptk2 ) or EV reported by GFP in retrovirally transduced BM cells isolated from C57BL/6 mice. FAK expression in preB cells transduced with EV (left) or FAK (middle). Representative histogram of preB cells transduced with FAK compared with proB cells transduced with EV (right). (B) pSTAT5a in EV- or FAK-transduced preB cells. (C) Frequency of EV- (left) or FAK-transduced (right) GFP Hi BM B-lineage cells and granulocytes from femur and tibia. (D) Overexpression of CXCR4 R334X (WHIM mutation) in retrovirally transduced BM cells isolated from RAG2:GFP knock-in mice. Transduced cell subsets reported by human CD4. CXCR4 expression in EV- (left) or CXCR4 R334X -transduced (right) preB cells gated on hCD4 Lo and hCD4 Hi cells. (E) BrdU incorporation in EV- (left) or CXCR4 R334X -transduced preB cells. Numbers indicate the frequency of BrdU + cells. (F) RAG2:GFP expression in gated hCD4 Hi and hCD4 Lo preB cells (EV, left; CXCR4 R334X , right). (G) Quantification of RAG2:GFP fusion protein geometric mean fluorescence intensity (GMFI). Circles depict individual mice. (H) Frequency of hCD4 Hi B-lineage cells and granulocytes in femur and tibia BM. Circles indicate mean ± SD ( n = 3). (C and H) Error bars indicate SD of three independent experiments. Numbers inside histograms (A, B, and D) indicate geometric mean fluorescence intensity ± SD ( n = 3 mice in each group). Data in all panels were pooled from three independent experiments. *, P < 0.05; **, P < 0.01 by unpaired Student’s t test (C and H). *, P < 0.05 by paired Student’s t test (G).

Article Snippet: Mouse monoclonal anti-human CD4 biotin , BD Biosciences , Clone RPA-T4; Cat#555345.

Techniques: Over Expression, Isolation, Expressing, Transduction, Mutagenesis, Knock-In, BrdU Incorporation Assay, Fluorescence

Journal: The Journal of Experimental Medicine

Article Title: Cell circuits between B cell progenitors and IL-7 + mesenchymal progenitor cells control B cell development

doi: 10.1084/jem.20180778

Figure Lengend Snippet: List of reagents, DNA constructs and mouse strains, sources, and catalog numbers

Article Snippet: Mouse monoclonal anti-human CD4 biotin , BD Biosciences , Clone RPA-T4; Cat#555345.

Techniques: Construct, Subcloning, Recombinant, Plasmid Preparation, Software